The Forth Road Bridge will open to traffic at 10pm as the Queensferry Crossing partially closes for snagging work.
Work on the £1.35 billion new bridge will include stripping back 15 metres of tarmac to resolve an issue around itsexpansion joints, described as a workmanship error.
It is anticipated the repairs will be complete at 6am on Wednesday December 6.
For the duration of the works southbound traffic will use the Forth Road Bridge and northbound traffic will use the Queensferry Crossing. A 40mph speed limit will be in place on both bridges.
Transport Scotland said signs and traffic management measures would be in place, and road users were advised to plan ahead.
The bridge has been built to carry traffic at 70mph but the national speed limit cannot be applied until snagging issues are resolved.
Traffic is currently restricted to a 50mph limit.
Transport Scotland said the bridge’s speed limit would be increased to 70mph by the end of December.
The snagging problems have been blamed for one motorist’s tyre blow-out.
Charline Smith, 44, said: “My tyre completely exploded, which led me to drive over the bridge petrified with only three wheels.
“I had my suspicion of what caused it as I was right at the joining of the bridge when it happened. But the news that the bridge is closing for repair confirmed it to me.”
The mechanic who repaired the wheel said there were no obvious signs of damage which would have caused the tyre to fail.
Brian Meston, of Altyre Services in Glenrothes, said: “The tyre itself had plenty of tread and there was nothing in the tyre like a nail. It looks like the tyre should have kept going.”
However, Richie Evans, who manages tyre firm Colin’s Inverkeithing, said: “We’ve had absolutely nothing, and we’re right on the bridge.
“Between myself and the guys, we’re going back and forth across the bridge at least 100 times a day.
“I would definitely put it down to coincidence.”
A Transport Scotland spokesman said: “We are unaware of any issue with the joints which would cause such implied damage at 50mph on the southbound joints.
“It is important to stress that there is no safety risk to those using the Queensferry Crossing, especially if they stick to the 50mph speed limit. Anyone experiencing difficulties on the bridge should use the hard shoulders and not continue to drive with a flat tyre.”