Perth and Kinross towns with a “high density” of traffic and pedestrian movements will have temporary 20mph signs put in place.
Villages in the region with limited or no footways will also see the temporary measure implemented.
The 41 temporary changes come as part of the £1.1million Spaces for People project, which supports active travel during lockdown.
Auchterarder, Alyth, Blairgowrie, Bridge of Earn, Comrie, Coupar Angus, Crieff, Glenfarg, Kinross, Milnathort, Pitlochry, and Scone will be affected.
Abernyte, Butterstone, Kirkmichael, and Spittalfield are among the 29 villages that will see the new limit put in place.
The council says the reduced speed limits will be implemented “in the coming weeks”.
A spokesperson for the local authority said: “Funded by the Scottish Government and managed by Sustrans Scotland, Spaces for People is a temporary infrastructure programme offering funding and support to make it safer for people who choose to walk, cycle or wheel for permitted journeys and exercise while physical distancing in place during Covid-19 as we transition out of lockdown.
“Work to install road signs for the 20mph speed limits will begin in the coming weeks.”
The move has been welcomed by local Crieff-based campaigner, Craig Finlay.
He said: “As someone who has been campaigning for a reduced speed limit through Crieff town centre, I warmly welcome the news and am delighted that it will now be rolled out.
“Pedestrian safety will be improved and those feelings of anxiety when walking the narrow pavements will ease for many people.
“Crieff town centre will be a more pleasant place to spend money.”
But Mr Finlay expressed his disappointment that the proposals do not extend to Crieff’s East High Street.
He added: “However, I was disappointed that East High Street has not been included in the proposal as there is strong local feeling that this area of town sees higher vehicle speeds than most.
“I’ll now have to continue to campaign for pedestrian crossing measures to be implemented in this busy area.”
The former chairman of Crieff Community Council had launched a petition towards the end of last year calling for increased safety measures on the town’s roads.
The petition, which received over 300 signatures, came after road safety concerns in the town were raised.
It also followed the death of an elderly man on its High Street, which campaigners say sees high volumes of fast-moving traffic.