Brussels cash is bankrolling £316 million worth of projects across Tayside and Fife, according to a new study.
A funding analysis by pro-EU campaigners shows how much the bloc has ploughed into areas including academic research, job creation and rural subsidies over the last few years.
The Best for Britain figures lay bare the scale of investment that Brexit is putting at risk, Labour and SNP politicians have warned.
But Leave supporters say money that has been sent to the EU – which when offset against cash coming the other way amounted to £9 billion in 2016 – can be spent in Britain instead.
Catherine Stihler, Labour MEP for Scotland and Best for Britain champion, said the data shows the “huge level of EU investment in Tayside and Fife, which is now at risk”.
“From farming to culture to research, the EU has boosted the region’s economy and created hundreds of local jobs,” the former St Andrews rector said.
“Brexit is now jeopardising people’s livelihoods, not to mention the wider impact from having less money for vital research.
“The only way through this mess is for a people’s vote with an option for the entire UK to remain in the EU.”
The data on the myeu.uk tool was pulled together from EU institutions and other public bodies, but researchers say the list is not exhaustive.
The biggest beneficiary of EU cash in Courier Country in 2017 was the farming industry, which received £97m in subsidies, according to the myeu.uk website.
The UK Government says those payment levels are guaranteed for three years after Britain leaves the Common Agricultural Policy in March 2019.
One of the largest EU grants in the area was £8.4m from 2014-2018 for Dundee University to accelerate the identification of new drug treatments for patients, as part of the European Lead Project.
Vote Leave claimed science and research funding would increase when UK contributions to the EU budget end.
Chancellor Philip Hammond has used his budgets to guarantee funding for EU projects agreed before the UK leaves.
John Swinney, the Perthshire MSP and Deputy First Minister, said his constituency has “benefitted hugely from the strong relationship between Scotland and the EU”.
“This is just the latest analysis which confirms that Brexit is going to inflict major harm on our economy,” he added.
“We are being dragged out of the European Union against our will, and Perthshire will be hugely damaged because of this.
“The only solution is to stay in the single market and customs union.”