Councils in Scotland face losing £46m a year in EU funding after Brexit, say the Scottish Liberal Democrats.
Brussels cash has helped Fife tackle poverty and funded job-boosting programmes in Angus since 2013/14, according to council figures obtained by the party.
Fife has received £1.25m a year on average from EU coffers over the last four years, compared with £425,000 in Angus and £212,250 in Perth and Kinross.
Dundee did not respond in time to the freedom of information request.
There is a further £6.1m that is ear-marked for councils in Tayside and Fife.
Tavish Scott, the Lib Dem MSP, said: “These figures reveal just how beneficial EU funding has been for council projects and the material threat posed by the UK’s departure from the EU.”
The UK Government says EU funds for structural and investment projects signed before the Autumn Statement, as well as Horizon research funding granted pre-Brexit, will be guaranteed by the Treasury after Britain leaves the EU.
A spokesman added: “Leaving the EU means we will be able to take our own decisions about how we deliver the policy objectives previously targeted by EU funding.”