Transport Scotland insists its £3 billion A9 dualling project remains on track, despite fresh opposition from Network Rail.
The rail company has lodged a formal objection against the Scottish Government’s road upgrade.
Network Rail is against the planned use of compulsory purchase powers to take over land beside the A9 at Perth, between Birnam and Luncarty.
Local landowners will also get the chance to voice their concerns at a hearing.
The Scottish Government’s department for planning and environmental appeals has confirmed the inquiry will be held at Bankfoot from June 8.
A Transport Scotland spokesman said project leaders were “committed” to having the A9 dualled by 2025, with work on the first phase between Kincraig and Dalraddy due to begin this summer.
In its written submission, agents for Network Rail stated it was against the company’s policy to allow its land to change hands via compulsory purchase powers.
A spokesman said the group wascontinuing to negotiate with Transport Scotland.
“Network Rail understands the importance of this scheme and thebenefits it will bring and wishes tosupport the scheme,” he said. “However, the scheme as proposed does prejudice its operational assets.”
The compulsory purchase order is also being contested by landowners, including Patrick Winton of Luncarty.
A spokesman said a proposed new access road could have a “considerable detrimental impact” on Mr Winton’s home.
He added that conditions duringconstruction would be “unbearable” for the household.
Agents for Westwood Farm, south of Bankfoot, said that a planned access route would have a “detrimental impact” on the farm’s operations: “affecting its viability and effectively severing the farm from its local community.”
Others who had originally objected to the CPO, have since withdrawn from the inquiry.