Roadworks are due to start on the A915 from Upper Largo on September 30.
The stretch of road running from Wester Newburn to Main Street in Upper Largo will be closed for up to six weeks for the carriageway resurfacing work.
Diversions will be in place.
Bus services will be affected and will follow the diversion route.
More information is available from Stagecoach on 01592 645680 or stagecoachbus.com.
Fife’s transportation convener, Labour councillor Altany Craik, apologised for any inconvenience and delays caused by the essential work.