A man has been taken to hospital following a crash at the A92 near Freuchie on Tuesday.
Multiple emergency services raced to the scene and the road was closed in both directions by police as the man was taken away for treatment.
Traffic Scotland asked users of the busy route, which connects Fife with Dundee, to travel a different way.
UPDATE❗️ ⌚️11:21#A92 RTC
CLOSED in both directions at Freuchie due to an RTC⛔️
— Traffic Scotland (@trafficscotland) February 15, 2022
Motorists also faced long tailbacks which built up in the area, and witnesses reported “numerous” emergency service vehicles, as well as an air ambulance.
The road has now been reopened, with traffic able to move through in both directions.
A spokesperson for Police Scotland said: “Around 10.30am on Tuesday, 15 February, police received a report of a two vehicle crash on the A92 near Freuchie, involving a car and an ambulance.
“One man was taken to hospital for treatment and the road was reopened around 1pm.”
Bus operators also reported disruption to services as a result of the crash.
Due to a RTA at Freuchie, service 36 will be unable to serve Freuchie and Newton of Falkland until further notice. Service will divert from Falkland via A912 to New Inn Roundabout. Sorry for any inconvenience this may cause pic.twitter.com/HQBUFdDh6e
— Stagecoach East Scotland (@StagecoachEScot) February 15, 2022
Stagecoach said its 36 service was unable to travel through Freuchie and Newton of Falkland until further notice.
The 42 bus diverted at Ladybank.